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High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

《化学科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 376-380 doi: 10.1007/s11705-015-1531-5

摘要： The Chinese Hamster Ovary (CHO K1) cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the production of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methionine sulfoximine. The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.

关键词： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

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Polycystic ovary syndrome

Zi-Jiang CHEN, Yuhua SHI

《医学前沿（英文）》 2010年 第4卷 第3期   页码 280-284 doi: 10.1007/s11684-010-0098-2

摘要： Polycystic ovary syndrome (PCOS) is a common gynecologic endocrinopathy. The pathogenesis of PCOS is associated with both heredity and environment. PCOS has adverse impacts on female endocrine, reproduction, and metabolism. PCOS can impact women’s reproductive health, leading to anovulatory infertility and higher rate of early pregnancy loss. PCOS has additional metabolic derangements, such as insulin resistance, impaired glucose tolerance, and dyslipidemia. The risks of diabetes, cardiovascular disease, hypertension, metabolic syndrome, and endometrial cancer among PCOS patients are significantly increased as well.

关键词： polycystic ovary syndrome     genetics     metabolic disturbance    

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基于就医人群的中国多囊卵巢综合征女性的临床表型和治疗结局—— 身体质量指数与地理分布的影响 Article

高敬书, 马红丽, 王宇, 杨新鸣, 曹义娟, 张蓓, 韩丛辉, 吴效科

《工程（英文）》 2021年 第7卷 第2期   页码 170-177 doi: 10.1016/j.eng.2020.12.006

摘要：

遗传因素、生活方式和环境因素与多囊卵巢综合征（PCOS）的发生相关。身体质量指数（BMI）的增加会加剧生殖及代谢障碍，降低PCOS女性的生育能力。本文对在中国大陆21个研究中心开展的一项大样本、多中心、随机对照试验进行二次分析。共有1000名PCOS女性参加本试验。其中，998名PCOS女性被纳入分析。BMI增加与月经失调加剧、高睾酮水平、高代谢综合征患病率以及生活质量降低相关。BMI正常组、超重组和肥胖组的女性人均排卵率分别为83.0%、78.2%和63.6%（P < 0.001），活产率分别为23.6%、18.1%和15.3%（P = 0.030）。与中国南方的PCOS患者相比，北方的PCOS患者生殖结局较差，血糖和血脂代谢不良，运动量少，以及总排卵率更低（81.2% vs 74.8%，绝对差：6.4%，95%置信区间：1.2%~11.5%）。结果显示，中国南北方的汉族PCOS女性具有典型的表型特征。与生活在中国南方的女性相比，在同样的干预措施下，中国北方的女性BMI更高，糖脂代谢障碍更严重，临床结局更差。表型特征及排卵的差异可以通过BMI差异加以解释。

关键词： 多囊卵巢综合征     体重指数     表型     中国队列     地理分布    

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Biopolymer-stabilized emulsions on the basis of interactions between β -lactoglobulin and ι -carrageenan

Qiaomei RU, Younghee CHO, Qingrong HUANG,

《化学科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 399-406 doi: 10.1007/s11705-009-0253-y

摘要： -Carrageenan and -lactoglobulin (-lg) stabilized oil-in-water (O/W) emulsions, which can be used for the oral administration of bioactive but environmentally sensitive ingredients, have been successfully prepared. The effects of protein/polysaccharide ratios, total biopolymer concentration, environmental stress (thermal processing and sonication), and pH on the complex formation between -carrageenan and -lactoglobulin have been investigated. We found that -lactoglobulin and-carrageenan stabilized emulsions can be formed at pH values of 6.0, 4.0, and 3.4. However, the microstructures of emulsions stabilized by -lactoglobulin and -carrageenan was identified by optical microscopy, and it indicated that the emulsion prepared at pH 6.0 flocculated more extensively, while its hydrodynamic radius was much bigger than those prepared at pH 4.0 and 3.4. Regarding rheological properties, the emulsion of pH 6.0 showed a more solid-like behavior but with a lower viscosity than those of pH 4.0 and 3.4. The optimum concentration ranges for -lg and-carrageenan to form stable emulsions at pH 4.0 and 3.4 were 0.3wt-%―0.6wt-% and 0.4wt-%―0.7wt-%, respectively.
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Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with

null

《医学前沿（英文）》 2017年 第11卷 第2期   页码 247-252 doi: 10.1007/s11684-017-0519-6

摘要：

The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.

关键词： in vitro fertilization     PCOS     free radical     embryo quality    

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Development of an axial-type fan with an optimization method

Chong-hyun CHO, Soo-yong CHO, Chaesil KIM,

《能源前沿（英文）》 2009年 第3卷 第4期   页码 414-422 doi: 10.1007/s11708-009-0038-5

摘要： An axial-type fan that operates at a relative total pressure of 671Pa and a static pressure of 560Pa with a flowrate of 416.6m/min is developed using an optimization technique based on the gradient method. Prior to the optimization of the fan blade, a three-dimensional axial-type fan blade is designed based on the free-vortex method along the radial direction. Twelve design variables are applied to the optimization of the rotor blade, and one design variable is selected for optimizing a stator which is located behind the rotor to support a fan-driving motor. The total and static pressure are applied to the restriction condition with the operating flowrate on the design point, and the efficiency is chosen as the response variable to be maximized. Through these procedures, an initial axial-fan blade designed by the free vortex method is modified to increase the efficiency with a satisfactory operating condition. The optimized fan is tested and compared with the performance obtained with the same class fan to figure out the optimization effect. The test results show that the optimized fan not only satisfies the restriction conditions but also operates at the same efficiency even though the tip clearance of the optimized fan is greater than 30%. The experimental and numerical tests show that this optimization method can improve the efficiency and operating pressures on axial-type fans.

关键词： axial-type fan     gradient method     free-vortex method     design variable     total pressure     static pressure     operating flowrate    

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Preliminary experimental study of a supercritical CO

Junhyun CHO, Hyungki SHIN, Jongjae CHO, Young-Seok KANG, Ho-Sang RA, Chulwoo ROH, Beomjoon LEE, Gilbong

《能源前沿（英文）》 2017年 第11卷 第4期   页码 452-460 doi: 10.1007/s11708-017-0504-4

摘要： Research on applying a supercritical carbon dioxide power cycle (S-CO ) to concentrating solar power (CSP) instead of a steam Rankine cycle or an air Brayton cycle has been recently conducted. An S-CO system is suitable for CSP owing to its compactness, higher efficiency, and dry-cooling capability. At the Korea Institute of Energy Research (KIER), to implement an S-CO system, a 10 kWe class test loop with a turbine-alternator-compressor (TAC) using gas foil bearings was developed. A basic sub-kWe class test loop with a high-speed radial type turbo-generator and a test loop with a capability of tens of kWe with an axial type turbo-generator were then developed. To solve the technical bottleneck of S-CO turbomachinery, a partial admission nozzle and oil-lubrication bearings were used in the turbo-generators. To experience the closed-power cycle and develop an operational strategy of S-CO operated at high pressure, an organic Rankine cycle (ORC) operating test using a refrigerant as the working fluid was conducted owing to its operational capability at relatively low-pressure conditions of approximately 30 to 40 bar. By operating the sub-kWe class test loop using R134a as the working fluid instead of CO , an average turbine power of 400 W was obtained.

关键词： supercritical CO2     power cycle     turbomachinery     compressor     turbine    

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Insight into the role of cholesterol in modulation of morphology and mechanical properties of CHO-K1

Lei Zhang, Lisha Zhao, Ping-Kai Ouyang, Pu Chen

《化学科学与工程前沿（英文）》 2019年 第13卷 第1期   页码 98-107 doi: 10.1007/s11705-018-1775-y

摘要：

Cholesterol plays a significant role in the organization of lipids and modulation of membrane dynamics in mammalian cells. However, the effect of cholesterol depletion on the eukaryotic cell membranes seems controversial. In this study, the effects of cholesterol on the topography and mechanical behaviors of CHO-K1 cells with manipulated membrane cholesterol contents were investigated by atomic force microscopy (AFM) technique. Here, we found that the depletion of cholesterol in cell membranes could increase the membrane stiffness, reduce the cell height as well as promote cell retraction and detachment from the surface, whereas the cholesterol restoration could reverse the effect of cholesterol depletion on the membrane stiffness. Increased methyl-β-cyclodextrin levels and incubation time could significantly increase Young’s modulus and degree of stiffing on cell membrane and cytoskeleton. This research demonstratede importance of cholesterol in regulating the dynamics of cytoskeleton-mediated processes. AFM technique offers excellent advantages in the dynamic monitoring of the change in membranes mechanical properties and behaviors during the imaging process. This promising technology can be utilized in studying the membrane properties and elucidating the underlying mechanism of distinct cells in the near-native environment.

关键词： cholesterol     methyl-β-cyclodextrin     atomic force microscopy     Young’s modulus     CHO-K1 cell    

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Effects of a dynamic membrane formed with polyethylene glycol on the ultrafiltration of natural organic matter

Boksoon KWON, Noeon PARK, Jaeweon CHO,

《环境科学与工程前沿（英文）》 2010年 第4卷 第2期   页码 172-182 doi: 10.1007/s11783-010-0002-y

摘要： The formation of a dynamic membrane (DM) was investigated using polyethylene glycol (PEG) (molecular weight of 35000 g/mol, concentration of 1 g/L). Two natural organic matters (NOM), Dongbok Lake NOM (DLNOM) and Suwannee River NOM (SRNOM) were used in the ultrafiltration experiments along with PEG. To evaluate the effects of the DM with PEG on ultrafiltration, various transport experiments were conducted, and the analyses of the NOM in the membrane feed and permeate were performed using high performance size exclusion chromatography, and the pore size distribution ( PSD) and molecular weight cut off ( MWCO) were determined. The advantages of DM formed with PEG can be summarized as follows: (1) PEG interferes with NOM transmission through the ultrafiltration membrane pores by increasing the retention coefficient of NOM in UF membranes, and (2) low removal of NOM by the DM is affected by external factors, such as pressure increases during UF membrane filtration, which decreases the PSD and MWCO of UF membranes. However, a disadvantage of the DM with PEG was severe flux decline; thus, one must be mindful of both the positive and negative influences of the DM when optimizing the UF performance of the membrane.

关键词： dynamic membrane     natural organic matters     ultrafiltration membrane performance     effective PSD     effective molecular weight cutoff    

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新型400 km·h−1 级架空接触网监测技术

Chul Jin Cho, Young Park

《工程（英文）》 2016年 第2卷 第3期   页码 360-365 doi: 10.1016/J.ENG.2016.03.016

摘要：

近年来，高速铁路各项技术不断发展，列车运行速度从300 km•h–1 提高到400 km•h−1。本文介绍了由韩国研究者开发和论证的技术，即400 km•h−1 级集电性能评估方法。此外，本文还详细解释了基于视频图像的监测技术，其不需要直接接触供电系统的任何部件，这项技术被应用于检测以时速400 km 运行的高铁上架空接触网组件的稳定性。与常规使用激光传感器或者线相机(line camera)来监控架空接触网的系统不同，开发的新型系统通过视频数据来测量其处于活动状态的参数。根据在商业线路上实地测量的结果，这种系统能有效地测量架空接触网的各项参数。

关键词： 高速铁路     架空接触网     状态监测     基于图像处理的测量方式    

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in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

《医学前沿（英文）》 2018年 第12卷 第5期   页码 518-524 doi: 10.1007/s11684-017-0575-y

摘要：

The increased levels of intracellular reactive oxygen species (ROS) in granulosa cells (GCs) may affect the pregnancy results in women with polycystic ovary syndrome (PCOS). In this study, we compared thein vitro fertilization and embryo transfer (IVF-ET) results of 22 patients with PCOS and 25 patients with tubal factor infertility and detected the ROS levels in the GCs of these two groups. Results showed that the PCOS group had significantly larger follicles on the administration day for human chorionic gonadotropin than the tubal factor group (P<0.05); however, the number of retrieved oocytes was not significantly different between the two groups (P>0.05). PCOS group had slightly lower fertilization, cleavage, grade I/II embryo, clinical pregnancy, and implantation rates and higher miscarriage rate than the tubal factor group (P>0.05). We further found a significantly higher ROS level of GCs in the PCOS group than in the tubal factor group (P<0.05). The increased ROS levels in GCs caused GC apoptosis, whereas NADPH oxidase 2 (NOX2) specific inhibitors (diphenyleneiodonium and apocynin) significantly reduced the ROS production in the PCOS group. In conclusion, the increased ROS expression levels in PCOS GCs greatly induced cell apoptosis, which further affected the oocyte quality and reduced the positive IVF-ET pregnancy results of women with PCOS. NADPH oxidase pathway may be involved in the mechanism of ROS production in GCs of women with PCOS.

关键词： PCOS     ROS     granulosa cell     IVF-ET     NADPH oxidase    

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Interfacial heat transfer coefficient between metal and die during high pressure die casting process of aluminum alloy

GUO Zhipeng, XIONG Shoumei, CHO SangHyun, CHOI JeongKil

《机械工程前沿（英文）》 2007年 第2卷 第3期   页码 283-287 doi: 10.1007/s11465-007-0049-z

摘要： The present work focused on the determination of the interfacial heat transfer coefficient (IHTC) between metal and die during the high pressure die casting (HPDC) process. Experiments were carried out on an aluminum alloy, ADC12Z, using step shape casting so-called because of its shape. The IHTC was successfully determined by solving one of the inverse heat problems using the nonlinear estimation method first used by Beck. The calculation results indicated that the IHTC immediately increased after liquid metal was brought into the cavity by the plunger and decreased as the solidification process of the liquid metal proceeded. The liquid metal eventually solidified completely, a condition when the IHTC tended to be stable. Casting thickness played an important role in affecting the IHTC between the metal and die not only in terms of its value but also in terms of its change tendency. Also, under the test conditions, different change tendencies of the metal solid fraction were found between castings with different thicknesses and the die.

关键词： so-called     calculation     inverse     interfacial     aluminum    

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美洲鲥鱼卵巢发育规律和性类固醇激素变化研究

洪磊,李兆新,陈超,郭正龙,王蔚芳,雷霁霖

《中国工程科学》 2014年 第16卷 第9期   页码 86-92

摘要：

采用组织学、形态学及电化学等技术方法，研究了人工养殖条件下美洲鲥鱼雌性亲鱼卵巢发育和性类固醇激素的周期变化规律。结果表明，美洲鲥鱼属于非同步分批产卵类型；根据卵巢外部形状大小及色泽等的不同，可将美洲鲥鱼卵巢发育过程分为6 个时期；根据卵母细胞的形态结构、卵黄物质的积累和滤泡细胞的变化，将卵子发生划分为6 个时相；卵巢周期变化过程中，肝脏指数（HSI）和肥满度（CF）变化不显著，性腺指数（GSI）与血浆中雌二醇（E2）水平表现出与性腺发育协同一致的变化规律。

关键词： 美洲鲥鱼     卵巢发育     卵子发生     组织学     形态学     性类固醇激素    

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Development of an integrated modeling system for improved multi-objective reservoir operation

Lei WANG, Cho Thanda NYUNT, Toshio KOIKE, Oliver SAAVEDRA, Lan Chau NGUYEN, Tran van SAP,

《结构与土木工程前沿（英文）》 2010年 第4卷 第1期   页码 47-55 doi: 10.1007/s11709-010-0001-x

摘要： Reservoir is an efficient way for flood control and improving all sectors related to water resources in the integrated water resources management. Moreover, multi-objective reservoir plays a significant role in the development of a country’s economy especially in developing countries. All multi-objective reservoirs have conflicts and disputes in flood control and water use, which makes the operator a great challenge in the decision of reservoir operation. For improved multi-objective reservoir operation, an integrated modeling system has been developed by incorporating a global optimization system (SCE-UA) into a distributed biosphere hydrological model (WEB-DHM) coupled with the reservoir routing module. The new integrated modeling system has been tested in the Da River subbasin of the Red River and showed the capability of reproducing observed reservoir inflows and optimizing the multi-objective reservoir operation. First, the WEB-DHM was calibrated for the inflows to the Hoa Binh Reservoir in the Da River. Second, the WEB-DHM coupled with the reservoir routing module was tested by simulating the reservoir water level, when using the observed dam outflows as the reservoir release. Third, the new integrated modeling system was evaluated by optimizing the operation rule of the Hoa Binh Reservoir from 1 June to 28 July 2006, which covered the annual largest flood peak. By using the optimal rule for the reservoir operation, the annual largest flood peak at downstream control point (Ben Ngoc station) was successfully reduced (by about 2.4m for water level and 2500m·s for discharge); while after the simulation periods, the reservoir water level was increased by about 20m that could supply future water use.

关键词： distributed biosphere hydrological model (WEB-DHM)     optimization     multi-objective reservoir     the Red River basin    

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Effects of heavy rainfall on the composition of airborne bacterial communities

Gwang Il Jang, Chung Yeon Hwang, Byung Cheol Cho

《环境科学与工程前沿（英文）》 2018年 第12卷 第2期 doi: 10.1007/s11783-018-1008-0

摘要： Wet deposition scavenges particles and particle-associated bacteria from the air column, but the impact of raindrops on various surfaces on Earth causes emission of surface-associated bacteria into the air column. Thus, after rainfall, these two mechanisms are expected to cause changes in airborne bacterial community composition (BCC). In this study, aerosol samples were collected at a suburban site in Seoul, Korea before and after three heavy rainfall events in April, May, and July 2011. BCC was investigated by pyrosequencing the 16S rRNA gene in aerosol samples. Interestingly, the relative abundance of non-spore forming operational taxonomic units (OTUs) was always higher in post-rain aerosol samples. In particular, the absolute and relative abundances of airborne always increased after rainfall, whereas those of airborne , including and , consistently decreased. Marine bacterial sequences, which were temporally important in aerosol samples, also decreased after rainfall events. Further, increases in pathogen-like sequences were often observed in post-rain air samples. Rainfall events seemed to affect airborne BCCs by the combined action of the two mechanisms, with potentially adverse effects on human and plant health.

关键词： Aerosol     Bacteria     Community composition     Pyrosequencing     Rain    

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标题 作者 时间 类型 操作

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

期刊论文

Polycystic ovary syndrome

Zi-Jiang CHEN, Yuhua SHI

期刊论文

基于就医人群的中国多囊卵巢综合征女性的临床表型和治疗结局—— 身体质量指数与地理分布的影响

高敬书, 马红丽, 王宇, 杨新鸣, 曹义娟, 张蓓, 韩丛辉, 吴效科

期刊论文

Biopolymer-stabilized emulsions on the basis of interactions between β -lactoglobulin and ι -carrageenan

Qiaomei RU, Younghee CHO, Qingrong HUANG,

期刊论文

Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with

null

期刊论文

Development of an axial-type fan with an optimization method

Chong-hyun CHO, Soo-yong CHO, Chaesil KIM,

期刊论文

Preliminary experimental study of a supercritical CO

Junhyun CHO, Hyungki SHIN, Jongjae CHO, Young-Seok KANG, Ho-Sang RA, Chulwoo ROH, Beomjoon LEE, Gilbong

期刊论文

Insight into the role of cholesterol in modulation of morphology and mechanical properties of CHO-K1

Lei Zhang, Lisha Zhao, Ping-Kai Ouyang, Pu Chen

期刊论文

Effects of a dynamic membrane formed with polyethylene glycol on the ultrafiltration of natural organic matter

Boksoon KWON, Noeon PARK, Jaeweon CHO,

期刊论文

新型400 km·h−1 级架空接触网监测技术

Chul Jin Cho, Young Park

期刊论文

in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

期刊论文

Interfacial heat transfer coefficient between metal and die during high pressure die casting process of aluminum alloy

GUO Zhipeng, XIONG Shoumei, CHO SangHyun, CHOI JeongKil

期刊论文

美洲鲥鱼卵巢发育规律和性类固醇激素变化研究

洪磊,李兆新,陈超,郭正龙,王蔚芳,雷霁霖

期刊论文

Development of an integrated modeling system for improved multi-objective reservoir operation

Lei WANG, Cho Thanda NYUNT, Toshio KOIKE, Oliver SAAVEDRA, Lan Chau NGUYEN, Tran van SAP,

期刊论文

Effects of heavy rainfall on the composition of airborne bacterial communities

Gwang Il Jang, Chung Yeon Hwang, Byung Cheol Cho

期刊论文